PROTEIN PILI 96,4 kDa Klebsiella pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN

Authors

  • Laila Rizqi Kurniawati Universitas Jember, Indonesia
  • M Ali Shodikin Departemen Mikrobiologi Fakultas Kedokteran Universitas Jember, Jember, Indonesia
  • Dini Agustina Departemen Mikrobiologi Fakultas Kedokteran Universitas Jember, Jember, Indonesia
  • Kristianningrum Dian Sofiana Departemen Fisiologi, Fakultas Kedokteran, Universitas Jember, Indonesia

DOI:

https://doi.org/10.24269/ijhs.v5i1.2700

Abstract

Klebsiella pneumoniae merupakan bakteri genus Klebsiella dalam famili Enterobactericeae. Klebsiella pneumoniae menyebabkan infeksi serius. Faktor virulensi penting dalam perlekatan awal bakteri untuk menginfeksi inang pili dan Outer Membrane Protein (OMP). Pili sebagai faktor virulensi dapat melekat kuat secara spesifik dan mampu menghindari pembersihan yang dilakukan oleh sel inang. Kemampuan adhesi suatu bakteri dipengaruhi oleh kemampuannya dalam mengagglutinasi eritrosit. Berdasarkan studi pendahuluan yang telah dilakukan, berat molekul protein pili yang muncul dari hasil elektroforesis SDS-PAGE yaitu 96,4 kDa memiliki pita tebal dan berkonsentrasi tinggi serta belum ada penelitian  membuktikan bahwa protein pili 96,4 kDa merupakan protein hemaglutinin dan adhesin.Tujuan penelitian ini untuk mengetahui peran protein pili 96,4 kDa bakteri Klebsiella pneumoniae sebagai protein hemaglutinin dan adhesin.  Penelitian ini dilakukan dengan mengisolasi protein pili Klebsiella pneumoniae kemudian dilakukan uji aglutinasi menggunakan sel eritrosit mencit dan uji adhesi menggunakan sel enterosit mencit. Hasil yang diperoleh dianalisis menggunakan Saphiro-Wilk. Hasil uji hemaglutinasi dengan menggunakan sel eritrosit mencit didapatkan titer hemaglutinasi paling tinggi untuk berat molekul protein pili 96,4 kDa adalah pada titer 1. Indeks adhesi dengan titer pengenceran memiliki hubungan yang bermakna yaitu  0,016 (p < 0,05) dengan nilai r = -0,847, sehingga disimpulkan protein pili 96,4 kDa Klebsiella pneumoniae merupakan protein hemaglutinin dan adhesin.

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Author Biography

Laila Rizqi Kurniawati, Universitas Jember

Fakultas Kedokteran Universitas Jember

References

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Published

2021-03-30

Issue

Vol. 5 No. 1 (2021): March

Section

Artikel

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